Introduction of HPLC Liquid Chromatography Instrument:
High performance liquid chromatography is a technique used in analytical chemistry for the separation, identification and quantification of each component in a mixture. The technique is based on the logic of advancing a pressure liquid solvent (mobile phase) containing a sample mixture from a column filled with a solid adsorbent (stationary phase) with high pressure.
Technical Parameters of HPLC Liquid Chromatography Instrument LC3400:
| Product Name | LC3400 Liquid Chromatography Instrument |
| Maximum Operating Pressure | 62MPa/75MPa |
| Flow Rate Setting Range | 0.0000～12.0000mL/min |
| Flow Rate Setting Increment | 0.0001mL/min |
| Gradient Error | ≤ 0.75% |
| Gradient Stability | 0.2% RSD (5% to 95%, 0.2 to 2.0 mL/min) |
| Flow Rate Setting Value Error | ±0.20% |
| Flow Rate Stability | ≤0.075% RSD |
| Autosampler | |
| Injection Volume | 100μL, 200μL, 500μL, 1000μL, 1500μL, 2000 μL |
| Repeatability | ＜0.3%RSD (2μL～Max), ＜1.0%RSD (0.1μL～1.9μL); |
| Linear Correlation Coefficient | >0.9999 |
| Number of Sample Position | 108 (2*54, 2mL), 96-well plates |
| Temperature Setting Range | 4.0～40.0℃ (optional refrigeration) |
| Temperature Setting Value Error | ±0.5℃ (optional refrigeration); |
| Temperature Stability | ±1℃ (optional refrigeration) |
| Column Oven | |
| Temperature Setting Range | 4℃-90℃ |
| Temperature Stability | ±0.03℃/h |
| Temperature Setting Value Error | ±0.1℃ |
| UV Detector | |
| Baseline Noise | ≤8.0×10-6 Au (1mL/min, methanol, C18) |
| Baseline Drift | ≤1.0×10-4 AU/h (1mL/min, methanol, C18) |
| Minimum Test Concentration | ≤5×10-9 g/mL (naphthalene/methanol) |
| Spectral Range | 188-900nm |
| Wavelength Error | ≤ ±1nm |
| Wavelength Repeatability | ≤ ±0.1nm |
| Linear Range | ≥1×104 or 2.5Au |
Features of HPLC Liquid Chromatography Instrument:
High Pressure Constant Current Pump Features:
1. Acetonitrile Resistant Check Valve: Adopting a new type of check valve greatly reduces the possible failure of the check valve when pure acetonitrile is used in the system.
2. Liquid leakage detection: Used for alarming in case of liquid leakage caused by misoperation, aging of pipes, etc;
3. Mixer: Titanium alloy mixer, gradient mixing more uniform.
Heating and Cooling Column Chamber (CT3400)
1. Dual-mode heating: heating-cooling dual-mode temperature control. Standard with semiconductor cooling module, resistance heating module.
2. Air bath: forced air bath circulation temperature control.
3. Temperature setting range: 4℃-90℃;
4. Temperature control range: room temperature -10 ℃ ~ room temperature +70.0 ℃ (not exceeding the maximum temperature, standard refrigeration module)
